Proceedings of the National Academy of Sciences · 1972 · 228 citations · 0 references
Natural SciencesBiophysicsPhysicsBiophysical ChemistryMolecular BiophysicsMolecular PhysicsBiophysical KineticsFluorescence SpectroscopyMolecular DynamicsMolecular RotationProtein DynamicsRotational StructureChemical Master EquationExponential DecayExponential Decay ModelsMaster Equation ApproachesMaster Equation ModelsMolecular Rigidity EffectsPolarizationRotational Dynamics
Reexamination of the theory of fluorescence time dependence owing to rotational diffusion of rigid macromolecules reveals deficiencies or hidden restrictions in each of the previous treatments. The correct master equation has five exponential decay terms, with preexponential factors that depend upon the diffusion constants and, in a completely symmetrical fashion, upon the orientations of absorbing and emitting dipoles.