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Use of Genetic Fingerprinting and Random Amplified Polymorphic DNA to Characterize Pathotypes of<i>Fusarium oxysporum</i>f. sp.<i>ciceris</i>Infecting Chickpea
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1994
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Genetic FingerprintingGeneticsPlant PathologyGenomicsPlant-pathogen InteractionFungal DiversityMolecular EcologyBiological TypingFungal BiologyCharacterize PathotypesSingle PrimersWilt SyndromesGenetic VariationPopulation GeneticsFungal PathogenBiologyMicrobiologyFungal SystematicsMedicine
Isolates of Fusarium oxysporum f. sp. ciceris induce either yellowing or wilt syndromes in chickpea and can be characterized into seven races by means of biological typing. DNA from 63 isolates of F. o. ciceris and from 11 isolates of other fungi was amplified by the random amplified polymorphic DNA (RAPD) technique by using the polymerase chain reaction with single primers. The primers used were based either on known ribosomal DNA sequences from Penicillium hordei or on sequencing primers. The amplification products were analyzed for polymorphisms by gel electrophoresis to determine whether pathotypes and/or races could be distinguished at the molecular level