Treatment of<i>Trillium Erectum</i>Prior to and During Mass Production of Permanent Smear Preparations

Abstract

Technics used in handling dormant plants of Trillium erectum L. prior to and during microsporogenesis are described. Normal meiosis occurs during storage of the plants at 4-6°C. Approximate times in days required for various stages of meiosis are presented. Schedules used for the mass production of aceto- or propiono-carmine smears of microsporocytes, microspores and pollen tubes are also given.