Differential expression analysis by cDNA-AFLP of Solanum torvum upon Verticillium dahliae infection

Abstract

Differentially expressed genes in eggplant (Solanum torvum SW) during root infection with Verticillium dahliae were identified using cDNA-amplified restriction fragment polymorphism (cDNA-AFLP) approach. A total of 140 differential transcript-derived fragments (TDFs) were isolated, in which 79 TDFs were up-regulated, and 34 TDFs were down-regulated after inoculation. BLAST analysis showed that 51% of these TDFs belonged to function-known genes. Further analysis on six TDFs (TDF11, TDF14, TDF16, TDF25, TDF26, and TDF39), which represented different expression patterns, was done. TDF11, encoding cytochrome c oxidase, appeared in 24 h after inoculation; TDF14, encoding retrotransposon protein, disappeared in 6 h after treatment; TDF16, encoding bZIP transcription factor, began to express only in 72 h after inoculation; TDF25, encoding senescence-associated protein, was present between 6 and 24 h; TDF26, encoding cytochrome P450 monooxygenase, and TDF39, encoding metallophosphoesterase, were up-regulated during the entire period after inoculation. Results obtained demonstrated that the defense of S. torvum against V. dahliae is a complex multigene process, which studying is helpful for better understanding verticillium wilt resistance of S. torvum.