Time‐resolved measurements of fluorescence from reaction centres of <i>Rhodopseudomonas viridis</i> and the effect of menaquinone reduction

Abstract

The kinetics of the fluorescence emitted by the ‘special pair’ of bacteriochlorophyll b molecules in reaction centres from Rhodopseudomonas viridis was recorded in the near infrared, with a time resolution of 1 ns. In nonreduced reaction centres two decay components were resolved with lifetimes of &lt;0.5 and 2.5 ns. Upon reduction of the menaquinone electron acceptor three decay components were detected with lifetimes of &lt; 0.5, 2.5 and 15ns.