Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon

Abstract

Strains JC15(T) and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H(2)S and showed beta-haemolysis. Strain JC15(T) grew optimally at pH 6 (range pH 5-8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30-37 degrees C (range 22-40 degrees C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B(12) was required as a growth factor by both strains. The major fatty acids were iso-C(15 : 0), C(17 : 1)omega8c and iso-C(13 : 0) 3-OH. The DNA G+C contents of strains JC15(T) and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15(T) and JC19 were most closely related to Shewanella haliotis DW01(T) (approximately 94 % sequence similarity) and to other members of the genus Shewanella. Genomic relatedness (DNA-DNA hybridization) between strains JC15(T) and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15(T) represents a novel species of the genus Shewanella, for which the name Shewanella fodinae sp. nov. is proposed. The type strain is JC15(T) (=CCUG 57102(T) =NBRC 105216(T) =KCTC 22506(T)).