Discrimination of three cyst-forming nematodes of the genus Globodera (Nematode: Heteroderidae) from Japan based on PCR-RFLP of ribosomal DNA

Abstract

The ITS1-5.8S-ITS2 region of rDNA sequences from three Globodera species found in Japan, G. rostochiensis, G. tabacum and G. hypolysi, were sequenced and compared. Sequence lengths for G. rostochiensis, G. tabacum and G. hypolysi were 1,001, 1,003 and 1,016 bp, respectively. Sequence divergence within the three Globodera species ranged from 3.8 to 14.7%. Eight restriction enzymes (AfaI, AluI, FspI, HhaI, HinfI, MvaI, PmaCI and SacII) were selected on the basis of sequence information. These three species were easily distinguished according to species-specific patterns by digesting the amplified products with AluI or PmaCI. The FspI-digested pattern distinguished G. rostochiensis from the other Globodera species.