Andrologia · 2009 · 49 citations · 6 references
Veterinary MedicineGynecologyObstetricsAssisted Reproductive TechnologiesInfertilityReproductive MedicineFertilization AssessmentIntracytoplasmic Sperm InjectionMale InfertilitySperm FunctionSperm Morphology AssessmentSperm Viability AssessmentFertilizationHypo-osmotic Swelling TestHypoosmotic Swelling TestIntracytoplasmic Sperm Injection FertilizationOsmotic SwellingSperm Function AssessmentSperm Function TestsSperm Selection Criteria
The success of intracytoplasmic sperm injection (ICSI) is poor when only immotile spermatozoa can be retrieved. In a couple with complete male asthenozoospermia the possible use of the hypo-osmotic swelling test to select spermatozoa for microinjection was examined. Following incubation in hypo-osmotic medium (Hypo 10, IVF Science, Göteborg, Sweden), 26% of immotile spermatozoa showed signs of sperm swelling (HOS-positive). After injection of HOS-positive spermatozoa, 5 out of 12 oocytes fertilized (41%) and after transfer of three embryos a healthy singleton pregnancy was achieved. In a previous ICSI cycle of this couple without preselection of spermatozoa by the HOS test, only 1 out of 10 oocytes fertilized. It is concluded that selection of spermatozoa by hypo-osmotic swelling-test prior to sperm microinjection seems to be a valuable tool to increase the fertilization rate in cases with complete asthenozoospermia.
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Rajasingam S. Jeyendran, H.H. van der Ven, M. Perez‐Pelaez et al. · Reproduction · 1984 · 1.7K citations · Full text