Monoclonal antibody to desmosomal glycoprotein 1—A new epithelial marker for diagnostic pathology

TLDR

Desmosomes are intercellular adhesive junctions present in almost all epithelia, making them promising epithelial markers for tumour diagnosis. The study introduces monoclonal antibody 32‑2B against the desmosomal glycoprotein dg1, intended for use in paraffin‑embedded tissue sections. The antibody was evaluated for its ability to stain epithelial cells and tumours in paraffin sections. 32‑2B stained all tested epithelia and a broad spectrum of carcinomas, including meningiomas, but failed to stain lymphomas, melanomas, sarcomas, or normal tissues lacking desmosomes, confirming its reliability as an epithelial marker for diagnostic histopathology.

Abstract

Abstract Desmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. Here, we describe a monoclonal antibody, 32‐2B, to a major desmosomal glycoprotein (dg1) which reacts with human tissues in paraffin sections. This antibody was tested for its ability to stain epithelia and tumours. It reacted with all epithelia tested and with every specimen of a wide range of carcinomas. It also stained meningiomas, another desmosome‐containing tumour. It did not stain other types of tumours including lymphomas, melanomas, and various sarcomas, or normal tissues which lack desmosomes. These characteristics demonstrate that 32‐2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology.