Journal of Biomedical Nanotechnology · 2013 · 32 citations · 0 references
EngineeringChemical EngineeringMaterials ScienceBiosensorsElectrochemistryNanotechnologyBiosensor DesignCholinergic SignalingElectrochemical BiosensorsElectrochemical SensorsNanomaterial-based BiosensorsNanosensorsElectrochemical BehaviorElectrochemical BiosensingElectrochemical MeasurementsElectrochemical NanobiosensorsLinear RangeNanomaterial-based SensorsNanomaterial-modified ElectrodesSelectivity
A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping beta-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATChI). It was shown that the modified nano-PPCPE displays a high selectivity and sensitivity. The acetylcholine biosensor showed the linear range from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M.