Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

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Methods in Enzymology , Vol

S P Colowick, M D Joshi, Jagannathan et al. · 1966 · 26.2K citations

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A simple method for displaying the hydropathic character of a protein

Jack Kyte, Russell F. Doolittle · Journal of Molecular Biology · 1982 · 23.1K citations

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A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

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Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Life Sciences, Microbiology, Molecular Biology +9