Biosynthesis of benanomicins.

Abstract

The biosynthesis of benanomicins A and B, produced by actinomycete strain MH193-16F4, was investigated by feeding experiments with 14C- and 13C-labeled compounds followed by measurement of radioactivity and 13C NMR analysis. A 2D double quantum coherence NMR spectrum of benanomicin A derived from sodium [1,2-13C2]acetate provided the location of intact acetate units, and confirmed the 13C assignments of benanomicins. The results indicate that the aglycone of benanomicins is derived from a dodecaketide, methionine and alanine.