Journal of Veterinary Medical Science · 1995 · 19 citations · 0 references
Veterinary MedicineInfectious DiseasesVeterinary MicrobiologyMolecular VirologyVaccinologyVirologyAnimal Infectious DiseasesAnimal VirusesAntigen Detection AssaysVeterinary ImmunologyVeterinary VaccinationVeterinary VirologyCanine Infectious DiseasesCanine VirologyExperimental Animal Models Of Viral InfectionInfectious Disease DiagnosticsNeutralizing Antibody ScreeningSerological ComparisonSerological IdentificationViral Antigen Detection
For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.