Flow‐injection analysis of ethanol with an alcohol dehydrogenase‐modified carbon past electrode

Abstract

Abstract An amperometric ethanol sensor based on carbon paste, chemically modified with alcohol dehydrogenase, nicotinamide adenine dinucleotide and Toluidine Blue O as a mediator, is described. The surface of the sensor is protected by covering with a dialysis membrane. Dialysis membranes with different molecular weight cut‐off were tested for preventing leaching of soluble species from the electrode. The influence of various experimental conditions was investigated for optimum analytical performance. Linear responses for ethanol in the range 2 × 10 −5 M to 2.4 × 10 −4 M at 0.05 V (Ag/AgCl) and a response time of 60 s were obtained. The sensor was also used in flow‐injection analysis and tested on wine samples.