Journal of Veterinary Medical Science · 2012 · 24 citations · 17 references
Veterinary MedicineMicrobiologyVeterinary MicrobiologyDairy MicrobiologyDiagnostic MicrobiologyMycologyFungal DiagnosticsMastitisMilk HygieneMilk MicrobiologyYeast BiologyYeasts In Dairy ProductsDairy-borne PathogensHygieneMastitis-associated MicroorganismsMolecular CharacterizationPolymerase Chain Reaction (Pcr) AmplificationVeterinary Mastitis MicrobiologyYeast Diversity In MilkYeasts In Milk
This study analyzed molecular-based identification of yeasts that associated with bovine clinical mastitis in Japan. Over 3,200 quarter milk samples from Holstein dairy cows collected in 2011 on Hokkaido and Honshu islands were examined. Yeast isolates were characterized by polymerase chain reaction amplification and sequencing of the D1/D2 region of the 26S rDNA. Molecular characterization confirmed that Candida spp. and Pichia spp. were most frequently isolated species. Our molecular analysis of mastitic milk samples demonstrated the prevalence of Pichia kudriavzevii(22/58) and Candida tropicalis(14/58). In addition, we demonstrated that molecular analysis of the D1/D2 region of the 26S rDNA is a rapid and reliable method for identifying clinically significant yeasts in dairy hygiene, including potentially new or emerging pathogenic species.
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Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text
Cletus P. Kurtzman, Christie J. Robnett · Journal of Clinical Microbiology · 1997 · 947 citations · Full text