In Vitro Systems for Genetic Recombination of the DNAs of Bacteriophage T7 and Yeast 2-micron Circle

Abstract

In the hope of gaining mechanistic insights into genetic recombination, we have focused our efforts on the development of systems that carry out this process in vitro. Two such systems are general recombination of bacteriophage T7 DNA promoted by T7-infected cell extracts (Lee and Sadowski 1983) and the site-specific recombination of the yeast 2-micron plasmid, catalyzed by the FLP protein of this plasmid (Vetter et al. 1983).