Publication | Closed Access
Resolution of the Optical Isomers of Underivatisized Amino Acids on Chemically Bonded Chiral Phases by Ligand Exchange Chromatography
72
Citations
25
References
1981
Year
EngineeringDirect SeparationOrganic ChemistryPeptide ScienceOptical IsomersChemistryUnderivatisized Amino AcidsSeparation ScienceLigand Exchange ChromatographyAnalytical ChemistryLiquid ChromatographyStereoselective SynthesisAmino Acid RacematesAdvanced SeparationChromatographyBiochemistryChromatographic AnalysisChiral Stationary PhaseAsymmetric CatalysisEnantioselective SynthesisBiomolecular EngineeringPeptide SynthesisMedicineDrug Analysis
Abstract A method for the direct separation of racemates by HPLC is described. A chiral stationary phase is synthesized, suitable for ligand exchange chromatography. L-proline is chemically bonded to silica gel via 3-glycidoxypropyltrimethoxysilane. The bonded support is loaded with Cu(II) ions as a complexing agent. Complete resolution of amino acid racemates can be obtained in less than ten minutes. α-values up to 3.5 are observed.
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