EXPERIMENTAL ALCOHOLISM IN RATS: PROTEIN SYNTHESIS IN SUBCELLULAR FRACTIONS FROM CEREBELLUM, CEREBRAL CORTEX AND LIVER AFTER LONG TERM TREATMENT¹

Abstract

Abstract— The incorporation in vivo of [ 3 H]leucine into protein from subcellular fractions was determined in rats chronically ingesting 15 per cent ethanol for 8 months. Mitochondrial, microsomal and cell sap fractions from cerebellum, cortex cerebri and liver were investigated. The results showed a minor over‐all depression of protein synthesis in cerebellum and cortex cerebri and a slight stimulation of the incorporation of leucine into protein from liver subcellular fractions. If the animals were abstinent 24 h before injection of the isotope, the incorporation of labelled amino acids into protein was markedly increased in cerebellum and cerebral cortex but not in liver.