Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of <i>Escherichia coli</i> by R-Factor DNA

Stanley N. Cohen, Annie Chang, Leslie Hsu · Proceedings of the National Academy of Sciences · 1972 · 2.7K citations

Life Sciences, Genetics, Microbiology +15

Functional messenger RNAs are produced by SP6<i>in vitro</i>transcription of cloned cDNAs

Paul A. Krieg, Douglas A. Melton · Nucleic Acids Research · 1984 · 1.5K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Multiple Mechanisms of Protein Insertion into and Across Membranes

William Wickner, Harvey F. Lodish · Science · 1985 · 717 citations

Life Sciences, Biochemistry, Molecular Biology +17