A rapid procedure for extracting genomic DNA from leukocytes

Simon W. M. John, Gabriel Weitzner, Rima Rozen, C R Scriver

Nucleic Acids Research · 1991 · 397 citations · 0 references

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Abstract

We have developed a rapid procedure based on standard protocols to isolate DNA from leukocytes. The procedure yields 100 µg to 150 µg from 5 ml of blood. The DNA produced is of good quality (fig. 1) and is suitable for restriction enzyme digestion or PCR amplification.