Serumless Medium for Cultivation of Cells of Normal and Malignant Origin.

Abstract

A serumless medium suitable for prolonged growth and repeated subculture of Walker carcinosarcoma 256, sarcoma 180, KB and human heart cells in vitro was described. In addition to amino acids, vitamins, minerals and glucose, it contained insulin, salmine sulfate, pyruvate, mucate, methyl oleate, folinic acid and lactalysate. The latter group of materials with exception of mucate was required for maximal growth rates, comparable to those on media containing dialyzed serum. Suspension cultures of Walker carcinosarcoma 256 were grown with continuous passage in the serumless medium.