Rhamnolipid Surfactant Production Affects Biofilm Architecture in <i>Pseudomonas aeruginosa</i> PAO1

TLDR

Biofilms are surface‑attached microbial communities that can develop complex three‑dimensional structures, and their architecture—particularly the spatial arrangement of colonies and surrounding open channels—is considered fundamental to their function. The study investigates a novel role for rhamnolipid surfactants produced by *Pseudomonas aeruginosa* in preserving biofilm architecture and proposes that this maintenance constitutes an unrecognized developmental step. The authors suggest that rhamnolipid synthesis is induced during late biofilm stages when cell density is high, indicating an active bacterial strategy that uses intercellular interactions and communication to sustain open channels. Mutants lacking rhamnolipid production fail to maintain noncolonized channels around macrocolonies, supporting the idea that surfactants help preserve these open spaces by modulating cell–cell interactions and surface attachment.

Abstract

In response to certain environmental signals, bacteria will differentiate from an independent free-living mode of growth and take up an interdependent surface-attached existence. These surface-attached microbial communities are known as biofilms. In flowing systems where nutrients are available, biofilms can develop into elaborate three-dimensional structures. The development of biofilm architecture, particularly the spatial arrangement of colonies within the matrix and the open areas surrounding the colonies, is thought to be fundamental to the function of these complex communities. Here we report a new role for rhamnolipid surfactants produced by the opportunistic pathogen Pseudomonas aeruginosa in the maintenance of biofilm architecture. Biofilms produced by mutants deficient in rhamnolipid synthesis do not maintain the noncolonized channels surrounding macrocolonies. We provide evidence that surfactants may be able to maintain open channels by affecting cell-cell interactions and the attachment of bacterial cells to surfaces. The induced synthesis of rhamnolipids during the later stages of biofilm development (when cell density is high) implies an active mechanism whereby the bacteria exploit intercellular interaction and communication to actively maintain these channels. We propose that the maintenance of biofilm architecture represents a previously unrecognized step in the development of these microbial communities.