Journal of Medical Microbiology · 2007 · 95 citations · 10 references
Veterinary MedicineBiologyInfectious DiseasesDiagnostic ParasitologyMedical MicrobiologyParasitologyMedical ParasitologyMolecular Diagnosis Of ParasitesParasitic Disease DiagnosisParasitic ZoonosesProtozoan ZoonosesZoonotic Parasitic DiseasesBlood Smear MicroscopyMicroscopic Parasite IdentificationParasite Species IdentificationPeripheral Blood Smear MorphologyPolymerase Chain Reaction (Pcr) AssaysTrypanosomatid-host InteractionsTrypanosome DetectionTrypanosome Infections
Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so parasite identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of Trypanosoma evansi and statistically different from those of Trypanosoma lewisi-like parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of approximately 623 bp that corresponded to the expected size for T. lewisi-like parasites. The ITS1 sequence from the infant's blood was 98 and 49 % identical to T. lewisi and T. evansi sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a T. lewisi-like (Herpetosoma) species.
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John O. Corliss, Cecil A. Hoare · Transactions of the American Microscopical Society · 1973 · 532 citations