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Production of Monoclonal Mouse IgE Antibodies with DNP Specificity by Hybrid Cell Lines
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1980
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ImmunologyHybrid Cell LinesPathologyImmunotherapeuticsMast CellMurine Myeloma CellsImmunotherapyImmunogeneticsDnp SpecificityImmunochemistryAutoantibodiesAntibody EngineeringAutoimmune DiseaseAutoimmunityPolyethylene Glycol FusionAntibody ScreeningCell BiologyImmunoglobulin EMedicine
IgE anti-DNP antibody-secreting hybridomas were obtained by polyethylene glycol fusion of murine myeloma cells with spleen cells from mice, primed with DNP-KLH and challenged with DNP-Nippostrongylus brasiliensis extract. The in vivo and in vitro continuously growing hybridomas are producing high amounts of monoclonal IgE anti-DNP antibodies, which are heat-labile, hapten-specific and have rat mast cell sensitizing capacity.