Implantation of rabbit embryo brain fragments into newborn mice: Integration and survival of xenogeneic astrocytes

Abstract

Brain fragments containing embryonic rabbit glia were implanted into the brains of newborn mice. The hosts developed an astroglial reaction around the transplants and along the needle tracks. Transplant-derived astrocytes were identified in the operated brain by their expression of rabbit GFAP. During the first few days post-implantation (PI) glial cells were exchanged between the transplant and the host. Less than 3 to 5 days PI, the transplant was extensively invaded by host astrocytes. Xenogeneic astroglial cells were first detected 10 days PI in the immediate proximity of the transplant. At 2 to 11 weeks, they could be detected either close to or at distance from the point of implantation. Most often, transplant-derived astrocytes presented a morphology similar to that of neighboring host astrocytes. Xenogeneic glial cells were found to participate in various types of astroglial features: sub-pial, pericapillary, fibrous, and protoplasmic. This morphological integration suggests that they are physiologically integrated, at least to a certain degree, in the host tissue. In spite of their integration into the host, xenogeneic astrocytes disappear after 3 months without signs of an inflammatory reaction.