Preparation of nanoparticles composed of chitosan and its derivatives as delivery systems for macromolecules

TLDR

The study prepared three types of nanoparticles for paracellular transport using a mild ionic‑gelation method. Chitosan, a chitosan–GTMAC blend, and quaternized O‑HTCC were cross‑linked with sodium tripolyphosphate, then characterized by TEM, AFM, PCS, and zeta‑potential, and loaded with bovine serum albumin. Increasing TPP increased chitosan nanoparticle size but decreased O‑HTCC size; O‑HTCC nanoparticles were slightly larger than pure chitosan but smaller than physical blends, and achieved 87.5 % loading efficiency and 99.5 % capacity with a burst‑then‑sustained release. © 2007 Wiley Periodicals, Inc., J Appl Polym Sci 2007.

Abstract

Abstract Three different kinds of nanoparticles for paracellular transport were prepared using a simple and mild ionic‐gelation method. Sodium tripolyphosphate (TPP) as crosslinking agent was added into three kinds of solutions, which were chitosan solution, physical blending solution of chitosan, and glycidyl trimethylammonium chloride (GTMAC), and O ‐(2‐hydroxyl) propyl‐3‐trimethyl ammonium chitosan chloride (O‐HTCC) solution respectively. O‐HTCC was synthesized by coupling of GTMAC to chitosan whose functional groups of the NH 2 groups were protected. The nanoparticles were characterized by transmission electron microscopy, atomic force microscopy, photon correlation spectroscopy, and zeta potential measurement. The results showed that increasing TPP concentration promoted the size of chitosan nanoparticles, a decrease in the size of O‐HTCC nanoparticles incurred on the contrary. The size of O‐HTCC nanoparticles is slightly bigger than that of pure chitosan nanoparticles, and smaller than that of physical blending nanoparticles (PBN). Bovine serum albumin (BSA), as a model protein drug, was incorporated into the nanoparticles. Compared with chitosan nanoparticles and PBN, high BSA loading efficiency (87.5%) and loading capacity (99.5%) are achieved by quaternized chitosan (O‐HTCC) nanoparticles, and the release profile of BSA from nanoparticles has an obvious burst effect and a slowly continuous release phase followed. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007