Journal of Forensic Sciences · 2005 · 14 citations · 1 references
Life SciencesGeneticsMolecular GeneticsGenomicsMedical GeneticsMolecular EpidemiologyMolecular TypingNucleic Acid Amplification TestingPolymerase Chain ReactionAmplified Fragment Length Polymorphism MarkersBrushesChelex-based Dna ExtractionDna Extraction From Oral SamplesHuman Dna QuantificationPolymerase Chain Reaction (Pcr) AmplificationPolymerase Chain Reaction (Pcr) AssaysReal-time Polymerase Chain Reaction (Pcr)
Abstract Buccal cells were collected by buccal brushes (Sterile Omni Swab, Whatman International Ltd.) from healthy, random Afghanian individuals. DNA was extracted by Chelex method (1). DNA samples were quantified using AmpFℓSTR® Quantifiler (Ap plied Biosystems, Foster City, CA) by Real time 7000 (Applied Biosystems, Foster City, CA), one nanogram of the samples obtained from the previous phase was amplified by GeneAmp® PCR System 9600 (Applied Biosystems, Foster City, CA). Simultaneous amplifications of 16 STRs loci (multiplexed PCR) were performed by using AmpFℓSTR® Identifiler™ (Applied Biosystems, Foster City, CA) according to the user manual recommendations (2).
1