Nanoscale · 2013 · 86 citations · 27 references
EngineeringBiochemistryMolecular BiologyBiophysical ChemistryBiosensorsNanomaterialsEnzyme-linked Immunosorbent AssayNanomaterial-based BiosensorsNanosensorsOptical BiosensingOptical BiosensorsPeptide ChemistryCleavageColor ShiftColorimetric BiosensorsNanoparticle-based BiosensorsNanoparticle-based SensingNanoparticlesPeptide-functionalized NanomaterialsProtease Cleavage Sites
A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL(-1)).
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Immunotargeted Nanoshells for Integrated Cancer Imaging and Therapy
Christopher Loo, Amanda Lowery, Naomi J. Halas et al. · Nano Letters · 2005 · 1.8K citations