Quantitative Determination of Guaifenesin, Phenylprolamine Hydrochloride Sodium Benzoate Δ Codeine Phosphate in Cough Syrups by High-Pressure Liquid Chromatography

Abstract

Abstract A rapid Paired-ion high-pressure liquid chromatographic method for the quantitative determination of guaifenesin, phenylpropanolamine hydrochloride, sodium benzoate and codeine phosphate in cough syrups was developed. Heptane sulfonic acid was used as a counter-ion in the mobile phase. A fixed wavelength detector (λ = 254 nm) and a μ-Bondapak phenyl column was employed. Total analysis time for all the active ingredients was 22 minutes.