Drug Development and Industrial Pharmacy · 2000 · 13 citations · 4 references
PharmacyPharmaceutical AnalysisPharmacologyChromatographic AnalysisPharmaceutical FormulationPharmaceutical TechnologyAnalytical Method ValidationChromatographic Analysis Of PharmaceuticalsChromatographic Method ValidationChromatographyLiquid ChromatographyMethod DevelopmentBioanalytical Sample PreparationChromatographic ValidationHigh-performance Liquid Chromatography Method DevelopmentInternal Standard AnalysisQuality Control AnalysisQuantitative Assay ValidationQuantitative Drug AssayReversed-phase Columns
A simple and accurate liquid chromatographic method was developed to estimate cyproterone acetate (CA) in pharmaceuticals. The drug was chromatographed on a reversed-phase C18 column. Eluents were monitored at a wavelength of 254 nm utilizing a mixture (60:40) of acetonitrile and water. Solution concentrations were measured on a weight basis to avoid the use of an internal standard. The method was statistically validated for linearity, accuracy, precision, and selectivity. Due to its simplicity and accuracy, we believe that the method can be used for routine quality control analysis. No specific sample preparation is required except for the use of a column guard and a suitable prefilter attached to the syringe.
4