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Optimization of silylation using N-methyl-N-(trimethylsilyl)-trifluoroacetamide, N,O-bis-(trimethylsilyl)-trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide for the determination of the estrogens estrone and 17α-ethinylestradiol by gas chromatography–mass spectrometry

Gas Chromatography–mass SpectrometryGas ChromatographyMedicineMass SpectrometryGynecologyEstrogens EstroneAnalytical ChemistryPharmacologyEndocrine ResearchSteroid MetabolismChromatographyDrug Analysis