Triplex Molecular Beacons as Modular Probes for DNA Detection

Abstract

Reporting live: Triplex formation is used to construct a stem–loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of “superquenched” beacons. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z700289_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.