Effect of Multiple Freeze and Thaw Cycles on the Sensitivity of IgG and IgM Immunoglobulins in the Sera of Patients With Syphilis

Abstract

We describe the effects of multiple freeze and thaw cycles on the sensitivity of the immunoglobulins IgG and IgM measured by enzyme-linked immunoassays in the sera of patients with syphilis. Stored frozen sera can withstand repeated freezing and thawing cycles with a minimal detrimental effect on the sensitivity of the sera.