Development of a scalable process for high‐yield lentiviral vector production by transient transfection of HEK293 suspension cultures

Abstract

Our process produces LV in suspension cultures and is consequently easily scalable, industrially viable and generated more than 10(11) total functional LV particles in a single bioreactor run. This process will allow the production of LV by transient transfection in sufficiently large quantities for phase I clinical trials at the 10-20-liter bioreactor scale.