Barometric Process Separation: New Method for Quantifying Nitrification, Denitrification, and Nitrous Oxide Sources in Soils

Abstract

Abstract A method (Barometric Process Separation, BaPS) was developed for the quantification of gross nitrification rates and denitrification rates in oxic soil using intact soil cores incubated in an isothermal gas tight system. Gross nitrification rates and denitrification rates are derived from measurements of changes (i) in air pressure within the closed system, which are primarily the result of the activities of nitrification (pressure decrease), denitrification (pressure increase), and respiration (pressure neutral), and (ii) of O 2 and CO 2 concentrations in the system. Besides these biological processes, the contribution of physicochemical dissolution of produced CO 2 in soil water to the pressure changes observed is to be considered. The method allows collection of additional information about the contribution of nitrification and denitrification to N 2 O emission from soil, provided simultaneous measurements of N 2 O emission are performed. Furthermore, BaPS can be used to quantify the percentage of N 2 O lost from nitrification. The advantage of BaPS is that disturbance of the soil system is minimized compared with other methods such as the use of gaseous inhibitors (e.g., acetylene) or application of 15 N compounds to the soil. We present the theoretical considerations of BaPS, results for nitrification rates, denitrification rates, and identification of soil N 2 O sources in a well‐aerated coniferous forest soil using BaPS. The suitability of BaPS as a method for determination of gross nitrification is demonstrated by validation experiments using the 15 N‐pool dilution technique.