A PPLO Slide Agglutination Test for the Detection of Infectious Sinusitis of Turkeys

Abstract

SINCE 1953, this laboratory has utilized the serum slide agglutination test for the detection of pleuropneumonia-like organism (PPLO) infections in turkeys. Jungherr et al. (1955) and Crawley and Fahey (1957) suggested that the hemagglutination inhibition (HI) and the tube agglutination tests were the most satisfactory to detect PPLO infection in poultry. Recently, Hofstad (1957) found that an antigen prepared from organisms grown in a basal chicken tissue medium was unsatisfactory for the detection of PPLO agglutinins in turkey sera by slide agglutination tests. The present paper reports the detection of PPLO infection in turkeys by the serum plate test and the use of this procedure in diagnosis and control of infectious sinusitis. MATERIALS AND METHODS The method of isolation of PPLO from chickens and turkeys was previously described (Adler et al., 1954) but a few modifications were used in this current study. The enrichments in the overlay fluid were 1% . . .