Antiviral, antioxidant and toxicological evaluation of mangrove plant from South East coast of India

Abstract

To identify the antiviral, antioxidant and toxicological evalution of the mangrove plant from South East coast of India. In vitro antiviral analysis such as RT inhibition assay, HBsAg binding assay, HBV DNA polymerase Inhibitory assays were carried out with the leaf extract of Avicennia marina, Avicennia officinalis, Rhizophora mucronata and Rhizophora apiculata mangrove plants. Further, the in vitro antioxidant assays and in vivo toxicological assay was also carried out with the most potent Avicennia marina leaf extract. Of the selected 4 mangrove plants, Avicennia marina leaf extract showed the minimum IC50 values with 403.91, 489.39 and 372.09 μg/mL concentrations against reverse transcriptase inhibition assay, HBsAg binding assay, HBV DNA polymerase Inhibitory assays, respectively. The antioxidant IC50 values were identified as 12.80±0.93, 640.06±34.93, 19.91±3.93 and 142.06±17.93 μg/mL concentrations against SOD, LPO, NO and DPPH assays, respectively. The phytochemical analysis of Avicennia marinaleaf extract showed the presence of reducing sugars, polyophenols, flavonoids and tannin. The LD50 value was 2 500 mg/kg bodyweight and the sub acute toxicity analysis did not show any significant changes in serum and haematological parameters when compared with the control animals. It can be concluded from the present findings that, the leaf extract of Avicennia marina can be further used as potential antiviral drug after completing the clinical trials.