Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251K citations

Protein Assembly, Natural Sciences, Structural Proteins +8

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Immunocytochemical Technique, Glycobiology, Polyacrylamide Gels +19

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Patricia S. Thomas · Proceedings of the National Academy of Sciences · 1980 · 8.4K citations · Full text

Engineering, Genetics, Ribosomal Rnas Transfer +19

[12] One-step gene disruption in yeast

Rodney Rothstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 3.3K citations

Genetics, Yeast, Molecular Genetics +2

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

David Botstein, S. Carl Falco, Sue Stewart et al. · Gene · 1979 · 960 citations

Engineering, Sterile Host Yeasts, Recombinant Dna Experiments +14