Concepedia

Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours

Shatha Adnan, Na Li, Hiroaki Miura, Yasuhiro Hashimoto, Hiroyuki Yamamoto, Takayuki Ezaki

FEMS Microbiology Letters · 1993 · 34 citations · 7 references

DOI

Concepts

Abstract

Single-stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA-DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.

References

7

Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains

T. EZAKI, Yohey Hashimoto, Eiko Yabuuchi · International Journal of Systematic Bacteriology · 1989 · 3.3K citations

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Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end

Søren Richard Rasmussen, Mette Ravn Larsen, Svend Erik Rasmussen · Analytical Biochemistry · 1991 · 174 citations

, , +13

Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells

Takayuki Ezaki, Yasuhiro Hashimoto, Noriko Takeuchi et al. · Journal of Clinical Microbiology · 1988 · 169 citations · Full text

, , +16

<i>Lactococcus piscium</i>sp. nov. a new<i>Lactococcus</i>species from salmonid fish

Annette M. Williams, J. L. Fryer, Matthew Collins · FEMS Microbiology Letters · 1990 · 118 citations · Full text

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<i>Streptococcus parasanguis</i>sp. nov., an atypical viridans<i>Streptococcus</i>from human clinical specimens

Robert A. Whiley, Hazel Y. Fraser, C.W.I. Douglas et al. · FEMS Microbiology Letters · 1990 · 94 citations

, , +15