The Journal of Clinical Endocrinology & Metabolism · 1971 · 13 citations · 0 references
Life SciencesBiochemistryEndocrinologyPediatric EndocrinologyReproductive EndocrinologySteroid BiochemistryAdrenal EndocrinologyAdrenal HormonesAdrenal Steroid HormonesAndrogen PhysiologySteroid Hormone BiochemistrySteroidogenesis Regulation17 Alpha-hydroxylaseAdrenal Androgen ProductionAdrenal AndrogensAdrenal Steroid Secretion DynamicsHormonal Biomarkers Of PubertyHormonal Markers Of PubertyPaper ChromatographyPlasma Steroid Profiling
ABSTRACT A shortened approach to the measurement of 17α-hydroxyprogesterone (17-OHP) is described. The extracted plasma sample with 3H indicator is purified by only one paper chromatography. No acetylation step or further chromatography is necessary. Values obtained in males and females are the same as reported by Strott et al. Both LH and FSH increased plasma 17-OHP in men. Measurable, but low, plasma values were observed in prepubertal children. Further evidence for adrenal secretion was provided by values from children and castrates, and by failure to suppress 17-OHP during the follicular phase with estrogen.