[Preparation of DNA by filtration on agarose gel].

Abstract

Abstract A technique of preparation of rat liver and calf thymus DNA is described. Chromatin is extracted by 2 M NaCl from nuclei prealably washed with buffer and filtrated through a gel of 4% agarose adjusted at the same ionic strength. The first peak yields highly polymerised DNA (as demonstrated by ultracentrifugation), very few proteins (