Cloning and sequencing of an α-glucosidase gene from Aspergillus niger and its expression in A. nidulans

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

Life Sciences, Biotechnology, Genetic Engineering +8

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

Steven Henikoff · Gene · 1984 · 4.2K citations

Life Sciences, Genetics, Molecular Biology +5

A complementation analysis of the restriction and modification of DNA in Escherichia coli

Herbert W. Boyer, Daisy Roulland-Dussoix · Journal of Molecular Biology · 1969 · 3.9K citations

Life Sciences, Genetics, Microbiology +11

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

Francisco Bolívar, Raymond L. Rodriguez, Patricia J. Greene et al. · Gene · 1977 · 3.9K citations

Life Sciences, Biotechnology, Genetic Engineering +9