Sensitive Ribonuclease Protection Assay Employing Glycogen as a Carrier and a Single Inactivation/Precipitation Step

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

Analytical Biochemistry · 1987 · 63.2K citations

Nucleic Acid Chemistry, Engineering, Natural Sciences +7

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Piotr Chomczyński, Nicoletta Sacchi · Analytical Biochemistry · 1987 · 46.8K citations

Engineering, Natural Sciences, Rna Isolation +7

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Protein Expression, Biochemistry, Single-step Purification +10

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Erich Hochuli, Heinz Döbeli, Alfred Schacher · Journal of Chromatography A · 1987 · 1.1K citations

Biochemistry, Natural Sciences, Peptide Engineering +7

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Chu di Guana, Ping Lib, Paul D. Riggsa et al. · Gene · 1988 · 671 citations

Engineering, Protein Expression, Biochemistry +11