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AN IMPROVED TECHNIQUE FOR MEASURING SOMATOMEDIN ACTIVITY IN VITRO
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1974
Year
Animal PhysiologyIncubation ApparatusMedicineBioanalysisPhysiologyOsteoarthritisSpecial DissectionChemical PathologyChromatographyPharmacologySomatomedin ActivityOsteoporosisDrug Discovery
ABSTRACT A method for measuring somatomedin activity in vitro is described. Porcine costal cartilage fragments are prepared and incubated using a special dissection and incubation apparatus. The schedule consists of a 24 h pre-incubation, 46 h of incubation with the test substance and 3 h of post-incubation in a medium containing both 35 S-Na 2 SO 4 and 3 H-thymidine. The average index of precision is 0.15 for 35 S-uptake and 0.19 for 3 H-thymidine. Although the assay is less sensitive than the hypophysectomized rat assay, it yields a considerable increase in precision, it is far less laborious to perform and is not so expensive.