Journal of the American Chemical Society · 2010 · 313 citations · 0 references
Natural SciencesBiophysicsCell BiologyBiomedical OpticsBiophotonicsPhotonicsCellular ImagingFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyMultiphoton MicroscopyOptical ImagingFemtosecond Laser PulsesMultiphoton Fluorescence ImagingMultiphoton Fluorescence MicroscopyTwo-photon Excitation MicroscopyTwo-photon Excited FluorescenceTwo-photon Fluorescence ImagingTwo-photon Fluorescence MicroscopyTwo-photon Microscopy
We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90-180 microm depth without interference from other biologically relevant species by two-photon microscopy.