<i>Sclerospora graminicola</i> Axenic Culture

Abstract

Sclerospora graminicola (Sacc.) Schroet., the obligate pathlogen causing downy mildew (green-ear) of pearl millet (Pennisetum typhoides Stapf. and Hubb.) has been successfully cultured for the first time on a known semisynthetic mediumn with no evident loss of fructifications. Sclerospora graminicola was first grown on host callus tissue and subsequently on a modification of White's basal medium that contained casein hydrolyzate (Oxoid). 2,4-dichlorophenoxyacetic acid, and kinetin.