Primary antibody formation <i>in vitro</i> by mouse cells in a complete homologous system

Abstract

Abstract Mouse lymphocytes have been cultivated in the absence of heterologous sera or mitogenic compounds. The fetal calf serum usually used was replaced by small amounts (0.5–1 mg protein/ml medium) of homologous exudate fluid induced by intraperitoneal injection of lysolecithin. Under these conditions, a primary immune response against various antigens (sheep red blood cells, dinitrophenyl‐dextran, dinitrophenyl‐human IgG) can be elicited. Adjuvant activity of lysolecithin has also been studied in this system.