Paraquat increases superoxide dismutase activity and radiation resistance in two mouse lymphoma L5178Y cell strains of different radiosensitivities.

Abstract

It is shown that paraquat treatment (1 h, 1 x 10(-5) mol/l) increases the activity of superoxide dismutase (SOD) in L5178Y (LY) R and S cells by about three times. When combined with X-irradiation, 0.5 h of treatment preceding irradiation increased the SOD activity two-fold and the alpha/beta ratio three-fold, as estimated from the X-ray survival curves. LY-S cells were more sensitive than LY-R cells to treatment with paraquat alone. These results indicate that SOD may be a radioprotective enzyme in LY strains and that LY-S cells are particularly sensitive to superoxide radicals as a result of a relatively low SOD activity. This explains their sensitivity to paraquat, which generates O2-, and to X-rays. The low SOD level may also explain the higher initial DNA damage in X-irradiated LY-S than LY-R cells.