Impedimetric Immunosensor for Detection of <i>Plum Pox Virus</i> in Plant Extracts

Abstract

Abstract This paper concerns the development of an immunosensor for detection of Plum pox virus in plant extracts. The sensor was based on gold electrodes modified with: 1,6‐hexanedithiol, gold nanoparticles, anti‐PPV IgG polyclonal antibody and BSA. It was used for determination of the virus in extracts from plum ( Prunus domestica ) and tobacco ( Nicotiana benthamiana ) leaves. The proposed electrochemical impedance spectroscopy (EIS) immunosensor displayed a very good detection limit of 10 pg/mL and a wide dynamic range from 10 pg PPV/ mL to 200 pg/mL. The presence of extracts from plant materials has no influence on the immunosensor’s response. The immunosensor was capable of discriminating between samples from healthy plants and samples containing 0.01 % of extract from infected plant material. The conditions for immunosensor regeneration are also presented.