Affinity‐tuned peroxidase‐like activity of hydrogel‐supported <scp>Fe₃O₄</scp> nanozyme through alteration of crosslinking concentration

Abstract

ABSTRACT In this work, we evaluated the effect of crosslinking concentration on the affinity of poly (2‐acrylamido‐2‐methyl‐1‐propansulfonic acid) (PAMPS) hydrogel‐supported Fe 3 O 4 nanozyme towards substrates (tetramethylbenzidine (TMB) and H 2 O 2 ). The peroxidase‐like catalytic activity of PAMPS/Fe 3 O 4 nanozyme was discussed with respect to crosslinking concentration of PAMPS hydrogel for the oxidation of TMB in the presence of H 2 O 2 at room temperature. High catalytic activity was achieved due to good dispersion of Fe 3 O 4 nanozyme in the hydrogel network and strong affinity of PAMPS hydrogel‐supported Fe 3 O 4 nanozyme towards substrates. The affinity between the hydrogel‐supported Fe 3 O 4 nanozyme and substrates can be improved by regulating the crosslinking concentration of PAMPS hydrogel without other trenchant experimental conditions. In addition, the result indicated that H 2 O 2 can be detected even at a concentration as low as 1.5 × 10 −6 mol L −1 with a linear detection range of 1.5–9.8 × 10 −6 mol L −1 . Such investigations not only showed a new approach to improve the affinity and peroxidase‐like activity of Fe 3 O 4 nanozyme, but also verified its potential application in bio‐detection and environmental chemistry. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43065.